Fluorescent enzyme immunoassay for thyroxine.
نویسندگان
چکیده
منابع مشابه
A sensitive manual enzyme immunoassay for thyroxine.
We describe an immunoassay for thyroxine in serum. In the assay specific antibody covalently bonded to latex particles is used, along with horseradish peroxidase as the label, and o-phenylenediamine as the chromogen. The flexible protocol is designed for manual execution. Performance is similar to that of the highest-sensitivity thyroxine radioimmunoassays. Results correlate well with radioimmu...
متن کاملAdaptations of enzyme immunoassay for thyroxine.
We expect and encourage narrow performance ranges within-laboratory, since it is here that the greatest control over laboratory error is possible. This is well illustrated by Gilbert (2), where he indicates that about two-thirds of the inter-laboratory error is attributable to within-laboratory performance characteristics. We thank Dr. Bogdan for his letter and are grateful for the opportunity ...
متن کاملFREE THYROXINE (fT4) ENZYME IMMUNOASSAY TEST KIT
Thyroxine, the principal thyroid hormone, circulates in blood almost completely bound to carrier proteins. The main carrier is thyroxine-binding globulin (TBG). However, only the free (unbound) portion of thyroxine is responsible for the biological action. Further, the concentrations of the carrier proteins are altered in many clinical conditions, such as pregnancy. In normal thyroid function a...
متن کاملRadioimmunoassay and automated enzyme immunoassay for the determination of thyroxine.
The EMIT homogeneous enzyme immunoassay for the determination of thyroxine (T4) was adapted to an automatic analyser (Type Kem-O-Mat) and compared with a conventional radioimmunoassay. Sera from 92 patients whose thyrometabolic status was being examined, and control sera with 3 different T4 concentrations were analysed by both methods. Day-to-day analyses of the control sera gave variation coef...
متن کاملContinuous-flow enzyme immunoassay for thyroxine in serum.
We have evaluated a fully automated thyroxine assay involving the use of a homogeneous enzyme assay and a Technicon AutoAnalyzer II continuous flow system. Comparison by regression analysis with a thyroxine radioimmunoassay kit method gave a slope of 0.82 and a y-intercept of 7.81 micrograms/L (SE 0.86 microgram/L, r = 0.95). Within-run precision yielded CVs of 1.0-6.1%, between-day CVs were 2....
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: BUNSEKI KAGAKU
سال: 1985
ISSN: 0525-1931
DOI: 10.2116/bunsekikagaku.34.9_544